Journal: Experimental cell research

Article Title: MMP-2 expression by fibroblasts is suppressed by the myofibroblast phenotype

doi: 10.1016/j.yexcr.2012.03.007

Figure Lengend Snippet: A, RDFs were grown on glass coverslips, serum-starved for 24 hours, then treated with serum-free medium or serum-free medium supplemented with 10% serum or 1 ng/ml TGF-β1. RDFs were fixed 24 hours later, and stained with phalloidin and anti-vinculin antibody. For A–C, values represent the mean +/− average deviation from the mean; significance was determined by unpaired, two-tailed Student’s t-test. Scale bar = 50 µm. B, RDFs were grown to confluence, and cultures were replenished with fresh serum-containing medium or serum-free medium. After 24 hours total RNA was isolated for analysis by real-time PCR. (n = 4) C, RDFs were cultured to confluence, serum-starved for 48 hours, then switched to fresh serum-free medium plus 1 ng/ml TGF-β. Total RNA was isolated after 24 hours for PCR analysis (n = 3). D, RDFs were cultured in attached collagen lattices for 5 days and either allowed to remain attached for 24 hours or released and allowed to float in media for 24 hours. Total RNA was isolated from RDFs in collagen lattices for PCR analysis (n = 6).

Article Snippet: Rat dermal fibroblasts (RDFs) were obtained from a 42-day old male Sprague-Dawley rat (Charles River Laboratories International, Inc., Wilmington, MA) using a primary explant technique [ 28 ] and stored in liquid nitrogen.

Techniques: Staining, Two Tailed Test, Isolation, Real-time Polymerase Chain Reaction, Cell Culture